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. Author manuscript; available in PMC: 2018 Aug 5.
Published in final edited form as: Biochem Biophys Res Commun. 2017 May 27;489(4):399–403. doi: 10.1016/j.bbrc.2017.05.151

Figure 3.

Figure 3

The effect of bicarbonate on respiration is cell-specific and observed across multiple respirometry instruments. (A) L6 myotubes were incubated with or without bicarbonate (10 mM) and respiration was assessed using the XFp Analyzer as described in Fig. 1A. Data presented as mean + SEM, from n = 4 separate experiments. (B) AML12 hepatocytes were incubated with or without bicarbonate (10 mM) and respiration was assessed using the XF24 Analyzer. Following basal measurements (Bas), cells were sequentially treated with insulin, oligomycin (Oligo), FCCP, and rotenone/antimycin A (Rot/AA). Data presented as mean + SEM, from n =3 separate experiments. (C) AML12 hepatocytes were incubated with or without bicarbonate (10 mM) and respiration was assessed using the O2K. Following basal measurements (Bas), cells were treated with FCCP. Data presented as mean + SEM, from n = 3 separate experiments. #,* p<0.05, using the one-tailed (#) or two-tailed (*) t-test.