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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Immunol. 2017 Jul 26;199(5):1886–1897. doi: 10.4049/jimmunol.1601066

Figure 3. TNF promotes ISC activation.

Figure 3

mRNA expression levels of stem cell markers and Wnt target genes (Lgr5, Ascl2, Ets, CD44, Axin2, Lrig, c-Myc and mTERT), differentitation markers (AP, Muc2, TFF3, Lys and CK20), and proliferation markers (Ki67 and PCMA) in (A) mouse small intestinal enteroids and (B) mouse colonoids cultured in the presence or absence of recombinant TNF for 5 days. The diameter (µm) of enteroids and colonoids are shown as insets. Enteroids and colonoids were cultured in individual experiments from 5 separate mice. For each experiment, 3–6 wells were treated for each condition. Values represent mean ± SEM, *p<0.01.