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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: J Microbiol Methods. 2012 Feb 2;89(1):71–75. doi: 10.1016/j.mimet.2012.01.016

Fig. 2.

Fig. 2

Time requirements by cPCR and rPCR—from sample collection to data acquisition. Schematic presentation of time required for each step in the detection of A. actinomycetemcomitans by rPCR as compared to cPCR. In the case of rPCR, it is necessary that all equipments and reagents be ready before beginning in order to complete quickly in 35 min. Notwithstanding the use of a quick DNA extraction method and a fast agarose gel electrophoresis system, cPCR takes approximately 2 h, which is still outside a “chairside” timeframe.