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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: J Microbiol Methods. 2012 Feb 2;89(1):71–75. doi: 10.1016/j.mimet.2012.01.016

Fig. 3.

Fig. 3

Detection of A. actinomycetemcomitans from human clinical samples by cPCR and rPCR. DNA purified from buccal samples from 40 subjects (20 each from A. actinomycetemcomitans-positive and A. actinomycetemcomitans-negative subjects) were used for PCR detection of A. actinomycetemcomitans. Panel A: cPCR; DNA was purified using the Qiagen kit, PCR performed on a regular PCR machine and PCR products were run on a conventional agarose gel. Panel B: rPCR; DNA was purified using QuickExtract™ solution, PCR performed on the AmpXpress rapid PCR machine, and PCR products were run on a 2% agarose gel in a bufferless agarose gel system for 6 min. Lanes: Data from 10 random A. actinomycetemcomitans-positive samples is shown. Lanes 1–10: LAP samples; lane 11: A. actinomycetemcomitans IDH781, used as positive control. Neg = negative control.