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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Mol Cancer Res. 2011 Feb 2;9(3):364–374. doi: 10.1158/1541-7786.MCR-10-0526

Figure 3. ESC8 induces autophagy in MDA-MB-231 cells.

Figure 3

a. The significant up-regulation of LC3B-II, starting with a 1 µM dose of ESC8, was detected, indicating a substantial increase in autophagy in MDA-MB-231 cells. The levels of Atg5-Atg12 complex and Beclin-1 did not change significantly upon ESC8 treatment. b. MDA-MB-231 cells were treated with ESC8 for 16 hours in presence or absence of pepstatin A and E64d. Processing of LC3-II was determined by Western blot analysis. c. LC3-II levels in MDA-MB-231 cells upon ESC8 treatment (5 µM) after Atg5 knockdown. d. ESC8-mediated killing was significantly reduced (* p< 0.01) after Atg5 knockdown in MDA-MB-231 cells. Figures represent three separate experiments with similar results.