|
| ||
| Compound | Amount per sample | Final concentration |
|
| ||
| DNA template | 1 μl | NA |
| F primer (20 μM) | 1 μl | 0.4 μM |
| R primer (20 μM) | 1 μl | 0.4 μM |
| 2× PCR mix | 25 μl | 1× |
| Water | Up to 50 μl | NA |
|
| ||
| NA, not applicable. | ||
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