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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Nat Protoc. 2014 Sep 4;9(10):2285–2300. doi: 10.1038/nprot.2014.081

Compound Amount per sample Final concentration

F primer (20 μM) 0.5 μl 0.5 μM
R primer (20 μM) 0.5 μl 0.5 μM
2× 2G Fast HotStart ReadyMix with loading dye 10 μl
Water up to 20 μl NA

NA, not applicable.