Figure 2.

ROTEM after escalating doses of AAV9/mouse FVIIa. (A) The diagram for the mouse FVIIa construct, flanked by AAV2 ITRs, with the TTR promoter, FVIII light chain, furin cleavage linker, FVIII heavy chain, and Poly A tail indicated. (B) Doses of AAV9/mFVIIa from 8 × 10¹¹ to 2 × 10¹³ particles/kg were administered into FVIII knockout mice via the tail vein. Whole blood was collected 6 weeks later from the inferior vena cava, and analyzed by ROTEM to measure: rotational thromboelastometry analysis of (a) α angle in degree, (b) clotting time, (c) clot formation time, and (d) maximum clot firmness. *p < 0.05 and **p < 0.01.