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. 2017 Aug 18;16(Suppl 1):65. doi: 10.1186/s12938-017-0356-5

Table 1.

Primers used for PCR amplification

Gene Direction Sequence
18S Forward 5′-TTT GAT GGT ACC TGC TAC TCG GAT AAC C
Reverse 5′-CTC TCC GGA ATC GAA CCC TAA TTC TCC
Ubiquitin monomer to pentamer Forward 5′-ATGCAGAT(C/T)TTTGTGAAGAC
Reverse 5′-ACCACCACG(G/A)AGACGGAG
Actin Forward 5′-TTG CTC TCG ACT ATG AAC AGG
Reverse 5′-CTC TCG GCC CCA ATA GTA ATA
Mitogen-activated protein kinase Forward 5′-CCAGGCGAGATTTCAGAGAC
Reverse 5′-TCGGTTTAAGGTCTCGATGG
Proline transporter Forward 5′-TTGTAGTGAGGGGCGGTTAC
Reverse 5′-CATGCAACCAAAGAAGCAGA
l-Ascorbate oxidase homolog Forward 5′-ACAAAAGGCATTGCTTGGTC
Reverse 5′-GGCCAAAACGAAGTTTACCA
Gliceraldehyde-3-phosphate dehydrogenase Forward 5′-GGGCAAGATCAAGATTGGAA
Reverse 5′-GTCTTCTCGCCGAACAAAAG
Salicylic acid-binding protein Forward 5′-GCATTGACCCGAAAATCCTA
Reverse 5′-AGGATGGCGGATTTGTAGTG
S-adenosylmethionine decarboxylase proenzyme Forward 5′-AGCTTCTGGCATCAGGAAAA
Reverse 5′-AGCCAGTACCCTCTCAAGCA