Table 2.
Bioinformatic miRNA target site predictions for corticosteroidogenic genes.
| Gene | 3′UTR length (base pairs) | MicroRNA.org | miRviewer | TargetScan | MicroCosm targets | Unique miRNAs with binding sites | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| CYP11B1 | 2022 | 151 | (142) | 4 | (4) | 436 | (333) | 33 | (30) | 390 |
| CYP11B2 | 1428 | 89 | (83) | 10 | (10) | 323 | (253) | 6 | (6) | 288 |
| CYP11A1 | 213 | 26 | (26) | 1 | (1) | 51 | (50) | 43 | (43) | 68 |
| CYP17A1 | 171 | 24 | (24) | 0 | 35 | (34) | 23 | (22) | 59 | |
| CYP21A2 | 508 | 57 | (56) | 11 | (10) | 78 | (70) | 25 | (22) | 134 |
| HSD3B2 | 414 | 68 | (63) | 0 | 73 | (68) | 46 | (44) | 125 | |
| StAR | 624 | 110 | (101) | 4 | (4) | 273 | (244) | 11 | (11) | 285 |
| HSD11B1 | 343 | 167 | (135) | 1 | (1) | 79 | (72) | 70 | (65) | 159 |
| HSD11B2 | 550 | 82 | (78) | 4 | (4) | 116 | (104) | 33 | (31) | 159 |
The 3′UTR lengths of these 9 genes were identified using the UCSC Genome Browser Gateway and Ensembl Genome Browser. The number of predicted miRNA target sites from each database is listed, and the number of unique miRNAs predicted to bind each is shown in parentheses. The final column shows the cumulative number of miRNA target sites, with duplicates removed.