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. 2017 Aug 9;2017:4297206. doi: 10.1155/2017/4297206

Table 1.

Primers sequences and enzymes used for the investigated polymorphisms.

Gene polymorphism Primers Restriction endonuclease
IL-10 -1082 G/A Fw 5′-CACTACTAAGGCTTCCTTGGGA-3′ XagI
Rev 5′-GTGAGCAAACTGAGGCACAGACAT-3′

TNF-α -308 G/A Fw 5′-TCCCCAAAAGAAATGGAGGCAATA NcoI
GGTTTTGAGGGCCAT-3′
Rev 5′-GAGACGTCTGCTGGCTGGGTG-3′

IL-6 -174 G/C Fw 5′-GCC TCA ATG ACG ACC TAA GC-3′ NlaIII
Rev 5′-TCA TGG GAA AAT CCC ACA TT-3′