Table 1.
Primer name | Sequence |
---|---|
F3-1 | AGTATCTACTACCACAATATCGG |
B3-1 | CCATGATGCTTGATGCAATA |
FIP-1 | AGTCGGATAAGGCAATTTTCCGTCTCATTGAAAGAGAAAGAGTTG |
BIP-1 | GTTCAAACACTTTGTGAAGCTCTGCACGTTCCGTAACTACCAT |
Loop-B | GGTCTTGCTAAAGCATTTCCTAGC |
F3-18 | ACITTCGGAAAATTGCCTTA |
B3-18 | ICTAAATGCAAGATTGTATTTCTCT |
FIP-18 | TACCATCATATTGCTAGGAAATGCTCCGACTCGCAATGTTCAA |
BIP-18 | GAAAGCTTATTGCATCAAGCATCAAICTACTCCCTCTAACTGT |
LoopA-18 | GCIAACAGAGCTTCACAAAGTGT |
LoopB-18 | TGGCACCACICAIGTGATGA |
F3-29 | TTATCCGACTCGCAATGT |
B3-29 | ICTAAATGCAAGATTGTATTTCTCT |
FIP-29 | CCGTAACTACCATCATATTGCTAGGCAAACACTTTGTGAAGCTCTG |
BIP-29 | CGTGAICAAAAAGAAAGCTTATTGCAICTACTCCCTCTAACTGT |
LoopA-29 | GCTTTAGCAAGACCATCAGCIAA |
LoopB-29 | ATCAAGCATCATGGCACCAC |
Primer sets compared against the ZEBOV alignment, any nucleotide that was divergent from the primer sequence in >1/143 sequences was replaced with an inosine residue. 3 primer sets with perfect identity to >99% of the ZEBOV isolates in the LANL alignment.