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. Author manuscript; available in PMC: 2018 Aug 7.
Published in final edited form as: Dev Cell. 2017 Aug 7;42(3):226–240.e6. doi: 10.1016/j.devcel.2017.07.001

Figure 3. Chromosome-Specific Alpha Satellite RNAs Have Array-Specific Size Ranges.

Figure 3

(A, A′) RNA FISH in RPE1 cells with PNA probe (green) recognizing the CENP-B box found in a subset of monomers within all alpha satellite arrays except for the Y. Multiple transcripts were detected in interphase (bar, 5μm), and at every centromere at metaphase (bar, 15μm). Asterisks denote HSAX.

(B) Northern blot detection of D17Z1 RNA in total RNA isolated from multiple cells lines, with each cell line in duplicate. Ethidium bromide stained gel is shown under each blot. Bracket indicates size range for chromosome-specific, array-specific transcripts. A schematic of the entire D17Z1 higher order repeat (HOR) that was used as a probe is shown.

(C) Northern blot of D17Z1-B RNA in multiple cell lines. A schematic of the entire D17Z1-B HOR that was used as a probe is shown.

(D) Northern blot of DXZ1 RNA. A schematic of the entire DXZ1 HOR that was used as a probe is shown.

(E) Northern blot of DXZ1 and D17Z1 RNA after rRNA depletion eliminated contaminating rRNA bands (*) observed in panels (B)–(D).