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. Author manuscript; available in PMC: 2018 Jun 19.

Published in final edited form as: Curr Protoc Nucleic Acid Chem. 2017 Jun 19;69:10.17.1–10.17.30. doi: 10.1002/cpnc.31

10-Fold scale up solid-phase purification of the phosphorothioate DNA sequence 12a. (A) RP-HPLC profile of unpurified 10a. (B) RP-HPLC profile of unpurified 10a after capture by the support 3. (C) RP-HPLC analysis of solid-phase purified 12a that has been released from the support 11a. (D) Photograph of precipitated 12a accounting for 995 OD₂₆₀ of the DNA sequence. Inset: Purity analysis of the solid-phase purified 12a by PAGE. Abbreviation: BP, bromophenol blue dye.

10-Fold scale up solid-phase purification of the phosphorothioate DNA sequence 12a. (A) RP-HPLC profile of unpurified 10a. (B) RP-HPLC profile of unpurified 10a after capture by the support 3. (C) RP-HPLC analysis of solid-phase purified 12a that has been released from the support 11a. (D) Photograph of precipitated 12a accounting for 995 OD₂₆₀ of the DNA sequence. Inset: Purity analysis of the solid-phase purified 12a by PAGE. Abbreviation: BP, bromophenol blue dye.