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. Author manuscript; available in PMC: 2018 Jul 24.
Published in final edited form as: Dev Cell. 2017 Jul 24;42(2):130–142.e7. doi: 10.1016/j.devcel.2017.06.022
Final Concentration Hybridization mix (μl)
Deionized formamide 10% 6
Competitior (4mg/ml E.coli tRNA + 5 mg/ml salmon sperm ssDNA) 1
Probe 1 10ng 2
Probe 2 10ng 2
20% Dextran Sulfate 10% 30
BSA (20mg/ml) 2mg/ml 5
20× SSC 2× 5
Vanadyl Ribonucleoside Complex (200mM) 10mM 2.5
ddH2O 6.5
Total volume 60