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Michaelis-Menten plots of diphenol oxidase activity of hTyrCtr (red), R422W (orange), R402Q (yellow), R422Q (green), and P406L (blue) as a function of L-DOPA concentration measured at 37°C. The lines represent nonlinear fits to the Michaelis-Menten equation obtained from OriginPro software. Experiments were performed in triplicate, and error bars represent the standard deviations.
