a, Relative MOE sensitivity of the sigM mutant and resistance of the ponA and Δ4 mutant compared to the wild type. Overnight cultures of each strain were diluted (10-fold series; left to right) and 10 µl spots were placed on NA containing MOE at 10 µg/ml. Right hand spots show the last three dilutions plated on NA with no MOE. sigM spottings were done in duplicate.
b, Synthetic lethality of a Δ4 mutant in a ΔsigM background. Growth on NA plates with or without 1 mM IPTG of strains AG221 (Δ4 pLOSS-Pspac-ponA) and AG831 (Δ4 pLOSS-Pspac-ponA
ΔsigM::kan) at 30°C.
c, Hypersensistivity of rodA, mreC and mreD mutants compared to wild type, ponA and Δ4 strains. Disc diffusion assays on MSM plates using 10 µg MOE per disc.
The experiments have been repeated several times during the course of the project, producing consistent results. The figures are representative of at least two independent experiments.