Table 3. Verification results of the ADx-SuperARMS EGFR assays.
Mutation | ΔCt value cut-off for positive calling | Mean ΔCt value from 0% of mutant in total DNA | Mean ΔCt value from 5% of mutant in total DNA | Mean ΔCt value from 1% of mutant in total DNA | Mean ΔCt value from 0.2% of mutant in total DNA | Mean ΔCt value from 0.1% of mutant in total DNA |
---|---|---|---|---|---|---|
E19Del | 11 | No ΔCt | 6.3 | 8.8 | 10.6 | 11.6 |
L858R | 11 | No ΔCt | 6.4 | 8.1 | 10.7 | 11.4 |
T790M | 8 | No ΔCt | 2.7 | 4.4 | 7.2 | 10.8 |
EGFR, epidermal growth factor receptor; E19Del: exon 19 deletion.