Table 2. Selected differentially abundant proteins in Potentilla fruticosa leaves.
| Accession | Unused | %Cov (95)a | Peptides (95%)b | Protein name | Species | Fold change (Heat vs Control) |
|---|---|---|---|---|---|---|
| Protein synthesis, folding and degradation | ||||||
| gi|470126953 | 5.12 | 4.93 | 1 | Heat shock 22 kDa protein | Fragaria vesca subsp. vesca | 27.29 |
| gi|470126676 | 9.71 | 13.95 | 5 | Chaperone protein ClpB1-like | Fragaria vesca subsp. vesca | 16.904 |
| gi|470129154 | 17.82 | 22.57 | 15 | Luminal-binding protein 5-like | Fragaria vesca subsp. vesca | 4.932 |
| gi|470101899 | 11.75 | 23.8 | 13 | Heat shock cognate 70 kDa protein-like | Fragaria vesca subsp. vesca | 3.996 |
| gi|470136963 | 14.4 | 34.44 | 9 | Chaperone protein ClpB3 | Fragaria vesca subsp. vesca | 24.262 |
| gi|470135104 | 2.67 | 7.94 | 1 | 26.5 kDa heat shock protein | Fragaria vesca subsp. vesca | 13.332 |
| gi|470116855 | 10.33 | 37.23 | 9 | 18.2 kDa class I heat shock protein-like | Fragaria vesca subsp. vesca | 29.651 |
| gi|470137715 | 12.37 | 18.68 | 6 | Peptidyl-prolyl cis-trans isomerase CYP38 | Fragaria vesca subsp. vesca | 0.387 |
| gi|659134080 | 8.33 | 34 | 4 | Heat shock protein 90–2, partial | Cucumis melo | 8.486 |
| gi|470147843 | 7.01 | 4.04 | 2 | Calnexin homolog 1-like | Fragaria vesca subsp. vesca | 3.771 |
| gi|470122167 | 5.39 | 11.48 | 2 | 60S ribosomal protein L13a-4-like | Fragaria vesca subsp. vesca | 0.331 |
| gi|590638131 | 5.82 | 18.71 | 2 | Translation protein SH3-like family protein | Theobroma cacao | 3.911 |
| gi|694380111 | 21.12 | 59.84 | 19 | Heat shock 70 kDa protein | Pyrus × bretschneideri | 15.955 |
| gi|470121863 | 0.26 | 0 | 1 | REVERSED: uncharacterized protein LOC101301382 | Fragaria vesca subsp. vesca | 0.649 |
| gi|645235988 | 22.89 | 22.82 | 17 | Elongation factor 1-alpha-like | Prunus mume | 1.556 |
| gi|225465198 | 20.16 | 27.47 | 8 | Elongation factor 1-gamma-like | Vitis vinifera | 1.706 |
| gi|470117806 | 6.44 | 35.59 | 4 | 30S ribosomal protein S1 | Fragaria vesca subsp. vesca | 0.466 |
| Abiotic stress defense and redox | ||||||
| gi|470110102 | 10.28 | 21.88 | 5 | Thioredoxin-like protein CDSP32 | Fragaria vesca subsp. vesca | 0.479 |
| gi|470121124 | 7.98 | 10.62 | 3 | Glutathione reductase | Fragaria vesca subsp. vesca | 1.941 |
| gi|470104065 | 2.85 | 6.62 | 1 | Uncharacterized protein LOC101298064 | Fragaria vesca subsp. vesca | 3.192 |
| gi|470121591 | 6.22 | 22.62 | 4 | Translationally-controlled tumor protein homolog | Fragaria vesca subsp. vesca | 4.04 |
| gi|470120512 | 17.69 | 18.73 | 7 | Linoleate 13S-lipoxygenase 2–1 | Fragaria vesca subsp. vesca | 0.069 |
| gi|470121668 | 3.03 | 6.75 | 1 | Major allergen Pru ar 1-like | Fragaria vesca subsp. vesca | 0.39 |
| gi|470133785 | 5.95 | 10.53 | 4 | Uncharacterized protein At2g37660 | Fragaria vesca subsp. vesca | 1.854 |
| gi|470127015 | 0.17 | 0 | 1 | Cytochrome P450 716B2-like | Fragaria vesca subsp. vesca | 18.727 |
| gi|470102448 | 10.74 | 32.9 | 7 | Peroxiredoxin Q | Fragaria vesca subsp. vesca | 0.27 |
| gi|694452607 | 0.11 | 0 | 1 | Beta-amyrin 28-oxidase-like | Pyrus × bretschneideri | 0.337 |
| gi|470117411 | 5.79 | 18.01 | 3 | NAD(P)H-quinone oxidoreductase subunit N-like | Fragaria vesca subsp. vesca | 0.207 |
| gi|470136232 | 9.87 | 16.3 | 5 | Uncharacterized oxidoreductase At1g06690 | Fragaria vesca subsp. vesca | 0.597 |
| Photosynthesis | ||||||
| gi|470102732 | 29.3 | 37.36 | 15 | Ferredoxin—NADP reductase | Fragaria vesca subsp. vesca | 0.259 |
| gi|470108717 | 18.23 | 22.83 | 8 | Chloroplast stem-loop binding protein of 41 kDa a | Fragaria vesca subsp. vesca | 0.495 |
| gi|470124625 | 0.17 | 0 | 1 | Protein CHUP1 | Fragaria vesca subsp. vesca | 0.175 |
| gi|428697270 | 19.48 | 40.17 | 17 | ATP synthase CF1 alpha subunit | Fragaria virginiana | 0.231 |
| gi|595817691 | 5.48 | 5.26 | 2 | Hypothetical protein PRUPE_ppa002248mg | Prunus persica | 1.629 |
| gi|470116673 | 4.01 | 13.38 | 2 | Pheophorbide a oxygenase, chloroplastic-like | Fragaria vesca subsp. vesca | 7.673 |
| gi|470108684 | 4.36 | 8.46 | 2 | 37 kDa inner envelope membrane protein | Fragaria vesca subsp. vesca | 0.325 |
| RNA metabolism | ||||||
| gi|470133931 | 0.45 | 0 | 1 | Serine/arginine-rich splicing factor RS2Z33-like | Fragaria vesca subsp. vesca | 1.857 |
| gi|470103932 | 9.07 | 3.4 | 3 | Splicing factor 3B subunit 3-like | Fragaria vesca subsp. vesca | 2.821 |
| gi|470110556 | 0.08 | 0 | 1 | Pentatricopeptide repeat-containing protein At1g71210-like | Fragaria vesca subsp. vesca | 1.738 |
| gi|470145892 | 0.14 | 0 | 1 | Pentatricopeptide repeat-containing protein At2g17525 | Fragaria vesca subsp. vesca | 0.515 |
| Signal transduction | ||||||
| gi|470129261 | 0.32 | 0 | 1 | REVERSED: rop guanine nucleotide exchange factor 1-like | Fragaria vesca subsp. vesca | 6.855 |
| gi|470112365 | 1.34 | 2.64 | 1 | Leucine-rich repeat and death domain-containing protein 1-like | Fragaria vesca subsp. vesca | 6.109 |
| gi|470120569 | 0.1 | 0 | 1 | REVERSED: protein tas-like | Fragaria vesca subsp. vesca | 14.862 |
| gi|470134540 | 0.19 | 0 | 1 | REVERSED: G-type lectin S-receptor-like serine/threonine-protein kinase SD3-1-like | Fragaria vesca subsp. vesca | 0.631 |
| Carbon metabolism | ||||||
| gi|470115774 | 15.61 | 7.15 | 6 | Phosphoenolpyruvate carboxylase, housekeeping isozyme-like | Fragaria vesca subsp. vesca | 1.888 |
| gi|470107435 | 4.6 | 14.12 | 2 | Isocitrate dehydrogenase [NAD] regulatory subunit 1 | NAD | 1.871 |
| gi|470127163 | 7.61 | 10.68 | 3 | Beta-glucosidase 44-like | Fragaria vesca subsp. vesca | 0.178 |
| DNA metabolism | ||||||
| gi|470132957 | 1.85 | 0.71 | 1 | FACT complex subunit SPT16-like | Fragaria vesca subsp. vesca | 4.613 |
| gi|470113538 | 0.1 | 0 | 1 | Protein FAR1-RELATED SEQUENCE 6-like | Fragaria vesca subsp. vesca | 13.428 |
| Transport | ||||||
| gi|470122886 | 0.43 | 0 | 1 | Putative clathrin assembly protein At2g01600-like | Fragaria vesca subsp. vesca | 10.375 |
| gi|470130490 | 0.09 | 0 | 1 | REVERSED: putative glycosyltransferase 5-like | Fragaria vesca subsp. vesca | 9.401 |
| gi|470135543 | 9 | 12.75 | 4 | Probable acetyl-CoA acetyltransferase | Fragaria vesca subsp. vesca | 0.581 |
a %Cov(95) represented the ratio of detected peptides (with 95% confidence) to the protein sequence.
b Peptides (95%) represented the total number of detected peptides (with 95% confidence) for the individual protein species.