Figure 5. EGFP uptake into HEK293T cells.

Representative histograms overlay from flow cytometric analysis of sample treated with 4µg EGFP complexed with PTDMs 1–4 (20/1 molar ratio). Complexes were added to subconfluent adherent cells in serum-containing media. After a 4 h incubation, cells were trypsinized and washed three times with a heparin solution (20 U/mL in PBS) to remove membrane-bound protein.