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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: J Control Release. 2017 Mar 29;254:131–136. doi: 10.1016/j.jconrel.2017.03.387

Figure 7. PTDM 1-mediated delivery of functional Cre Recombinase into LoxP-GFP reporter human T cell line.

Figure 7

a) Overlay of histograms from flow cytometric analysis showing human reporter T cells treated with 150 nM of Cre Recombinase alone (black line) or in a non-covalent complex with 1 (red line) and 2 (blue line). Quantification of target gene recombination induced by increasing concentration of active Cre delivered into the reporter T cells by b) PTDM 1 and c) PTDM 2. The molar ratio used for complex formation was 10/1 (PTDM/Cre). Delivery was conducted for 2h in serum-free media and cells were analyzed by flow cytometry for gene recombination 72h after treatment and compared to an untreated sample. Values and error bars represent the mean ± SEM of at least three independent experiments.