Table 1.
Optimized MS parameters for nine hydroxylated PAH metabolites.
| No. | Analyte | Abbreviation | Ion Transition (m/z) | IS Ion Transition (m/z) | Collision Energy (eV) | Parent PAH |
|---|---|---|---|---|---|---|
| 1 | 1-hydroxynaphthalene | 1-OH-NAP | 143→115 | 149→121 | −34 | Naphthalene |
| 2 | 2-hydroxynaphthalene | 2-OH-NAP | 143→115 | 149→121 | −34 | |
| 3 | 2-hydroxyfluorene | 2-OH-FLU | 181→180 | 187→186 | −34 | Fluorene |
| 4 | 3-hydroxyfluorene | 3-OH-FLU | 181→180 | 187→186 | −26 | |
| 5 | 1-hydroxyphenanthrene | 1-OH-PHE | 193→165 | 197→168 | −38 | Phenanthrene |
| 6 | 2-hydroxyphenanthrene | Σ2,3-OH-PHE* | 193→165 | 199→171 | −41 | |
| 7 | 3-hydroxyphenanthrene | |||||
| 8 | 4-hydroxyphenanthrene | 4-OH-PHE | 193→165 | 197→168 | −38 | |
| 9 | 1-hydroxypyrene | 1-OH-PYR | 217→189 | 223→195 | −45 | Pyrene |
*
2-OH-PHE and 3-OH-PHE are measured together.