Table 3.
Accuracy for the measurement of hydroxylated PAH metabolites.
| Analyte | Level -1 (n=7) | Level -2 (n=7) | Level -3 (n=7) | SRM 3672 Smoker urine (n=4) | SRM 3673 non-Smoker urine (n=4) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Spiked (ng/mL) | Accuracy | Spiked (ng/mL) | Accuracy | Spiked (ng/mL) | Accuracy | Measured (ng/mL) | NIST Certified (ng/mL) | Accuracy | Measured (ng/mL) | NIST Certified (ng/mL) | Accuracy | |
| 2-OH-NAP | 0.71 | 112.8±4.3% | 3.85 | 99.7±1.6% | 19.23 | 99.7±0.8% | 8.88±0.17 | 8.73 | 101.7±2.0% | 1.43±0.07 | 1.35 | 106.3±4.8% |
| 1-OH-NAP | 0.70 | 113.3±1.9% | 3.83 | 95.3±1.2% | 19.13 | 98.7±1.2% | 36.85±0.68 | 34.4 | 107.7±2.7% | 207.38±3.94 | 211.00 | 98.3±1.87% |
| 3-OH-FLU | 0.17 | 107.2±4.2% | 0.91 | 98.6±2.6% | 4.57 | 98.1±2.0% | 0.41±0.02 | 0.43 | 96.8±4.7% | 0.04±0.01 | 0.04 | 112.0±25.6% |
| 2-OH-FLU | 0.18 | 107.4±2.3% | 0.96 | 99.3±1.3% | 4.78 | 100.3±1.4% | 0.72±0.04 | 0.87 | 82.8±4.6% | 0.09±0.01 | 0.11 | 83.7±9.3% |
| Σ2,3-OH-PHE | 0.36 | 113.1±1.8% | 1.94 | 99.4±1.8% | 9.68 | 101.9±1.2% | 0.20±0.02 | 0.21 | 96.1±9.6% | 0.05±0.01 | 0.05 | 103.0±18.9% |
| 1-OH-PHE | 0.18 | 109.8±2.8% | 0.95 | 99.4±2.8% | 4.75 | 101.2±2.4% | 0.11±0.01 | 0.14 | 84.3±7.4% | 0.05±0.01 | 0.05 | 98.4±20.4% |
| 4-OH-PHE | 0.17 | 108.5±3.2% | 0.94 | 98.3±3.2% | 4.7 | 98.2±1.2% | 0.05±0.01 | 0.05 | 94.2±20.4% | * | 0.01* | * |
| 1-OH-PYR | 0.18 | 105.5±5.0% | 0.97 | 93.6±2.6% | 4.86 | 101.8±2.6% | 0.18±0.02 | 0.17 | 102.2±11.6% | * | 0.03* | * |
*
Results not reported because NIST certified values are close or below the method LOD for these analytes.