Skip to main content
. Author manuscript; available in PMC: 2018 Jun 29.
Published in final edited form as: Cell. 2017 Jun 22;170(1):142–157.e19. doi: 10.1016/j.cell.2017.06.007

Figure 3. CD8 T cell-Intrinsic De Novo Programming Regulates Exhaustion.

Figure 3

(A) Experimental setup for tracking congenically distinct WT and cKO P14 CD8 T cells in LCMV infected Rag1 KO mice.

(B) Longitudinal measurement of WT and cKO P14 cell frequencies in the peripheral blood of chimeric Rag1 KO mice during chronic LCMV infection.

(C) Representative FACS analysis and summary graphs of cKO (red) and WT (blue) P14 cell frequency in tissues of chronically infected chimeric Rag1 KO mice at 35 dpi.

(D) Representative FACS analysis and summary graph of IFNγ expression frequency among cKO and WT P14 cells from spleens of chronically infected chimeric Rag1 KO mice.

(E) Representative FACS analysis and summary graphs of intracellular TCF1 and Ki67 levels among cKO and WT P14 cells in spleens from chronically infected chimeric Rag1 KO mice.

(F) Representative post-sort purity FACS analysis of cKO and WT P14 cells from spleens of chronically infected chimeric Rag1 KO mice.

(G) Locus-specific DNA methylation analysis of the IFNγ and Tcf7 DMRs in naïve P14 cells from uninfected mice, and WT and cKO P14 splenocytes from chronically infected chimeric Rag1 KO mice.

N = 3 mice per group of two independent experiments. Error bars indicate SEM.