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Summary of 11-cis Retinol Binding to X2IRBP and Its Tryptophan to Phenlyalanine Substitution Mutants
| Protein | Sites (N) Kd (μM) |
Enhancement | Quenching |
|---|---|---|---|
| X2IRBP | N | 0.95 ± 0.03 | 0.93 ± 0.02 |
| Kd | 0.077 ± 0.015 | 0.042 ± 0.009 | |
| W450F | N | 1.00 ± 0.06 | |
| Kd | 0.218 ± 0.062 | —* | |
| W587F | N | 1.67 ± 0.06 | 0.77 ± 0.02 |
| Kd | 0.132 ± 0.056 | 0.024 ± 0.010 |
No significant quenching was observed.
