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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: Sci Signal. 2015 Nov 10;8(402):ra114. doi: 10.1126/scisignal.aab2677

Figure 2. Biophysical characterization of selected IL-13 agonists.

Figure 2

(A) Binding isotherms of the IL-13 agonists displayed on the surface of yeast. Yeast-displayed IL-13 agonists were incubated with the indicated concentrations of biotinylated IL-13Rα1 ECD, stained with Alexa-647-coupled Streptavidin, and assessed via flow cytometry. Sigmoidal curves were fitted using Prism software (GraphPad). (B) Normalized KD binding affinities of the recombinant IL-13 agonists determined via surface plasmon resonance (SPR). The IL-13 KD was fixed to one and other agonists were normalized accordingly. (C) Summary of the amino acids mutated in the IL-13 agonists as well as the kon, koff and KD binding parameters values obtained from SPR experiments.