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. Author manuscript; available in PMC: 2017 Aug 23.
Published in final edited form as: J Immunol. 2016 Nov 16;198(1):257–269. doi: 10.4049/jimmunol.1601200

FIGURE 2.

FIGURE 2

Deletion of Bim at the early DP stage promotes the accrual of TCR+ DN thymocytes. (A) Thymocytes from Bimf/f or CD4Cre+Bimf/f mice (n = 3) were stained with Abs and analyzed by flow cytometry. Numbers in representative dot plots show the frequency (percentage) of corresponding populations. The bar graphs show the frequency and cell numbers of each population from either Bimf/f (open bars) or CD4Cre+Bimf/f (filled bars) mice. (B) Histograms show the percentage of CD25−CD44− DN thymocytes that express surface TCRβ-chain. (C) Bar graph shows the percentage of CD25−CD44− DN thymocytes that express the surface TCRα-chain (a combination of Vα2, Vα3.2, Vα8.3, and Vα11) from either Bimf/f (open bars) or CD4Cre+Bimf/f (filled bars) mice. (D) Data in representative histograms show Bim levels in various gated thymic subpopulations, and the bar graphs show the frequency of Bim+ cells in thymic subpopulations from either Bimf/f (open bars) or CD4Cre+Bimf/f (filled bars) mice. Results are representative of at least three independent experiments and show mean ± SD. **p < 0.01.