Melatonin up-reguated prohibitin concentration and promotednuclear localization of prohibitin in ARPE-19 cells. ARPE-19 cells were fluorescently labeled with DAPI, MitoTracker Orange, and AlexaFluor488 to show the nucleus (blue), mitochondria (red) and prohibitin (green), in melatonin treatment (100 μM, A, B, C) versus control (D, E, F). Prohibitin localization is up-regulated in the nucleus and prohibitin concentration is increased. Mitochondria was altered into ring-orientated structure.