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(a) Scheme for the formation of Oligo functionalized monomeric (upper) and multimeric (lower) nanostructures. DHFR2RGD4C contains two DHFR proteins (gray) and cRGD peptide (blue ring with 3 red dots). Bis-MTX-ASO has a bis-MTX, shown in green, attached to the ASO (blue). Size exclusion chromatography (SEC) trace of 13DHFR2RGD4C (b) or 1DHFR2RGD4C (c) alone (black) and after incubation with bis-MTX-ASO (red)
