. Author manuscript; available in PMC: 2017 Aug 23.

Published in final edited form as: Nat Neurosci. 2015 Mar 3;18(5):637–646. doi: 10.1038/nn.3980

Table 1. Cell populations immunolabeled and collected by FACS from human fetal cortex for RNA-seq analysis.

Dissociated fetal human cortical tissue was immunolabeled for radial glial progenitor markers LeX (CD15), GLAST (SLC1A3), and PROM1 (Prominin; CD133). LeX and Glast are detected on the vast majority of radial glial cells^16–18, while Prom1 is specific to the apical membrane domain found at the ventricular surface^19,20.

Sorted Pool	Antigenicity	Enriched Markers (qRT-PCR)	Cell Type
LG⁺Pr^hi	LeX⁺, Glast⁺, Prom1-high	PAX6, SOX2, VIM, NES, BLBP, PARD3 (PAR3), TJP1 (ZO1), MPP5 (PALS)	apical radial glial cells
LG⁺Pr^lo	LeX⁺, Glast⁺, Prom1-low	PAX6, SOX2, VIM, NES, BLBP	non-apical radial glial cells, including ORG
LG⁻Pr⁻	LeX⁻, Glast⁻, Prom1⁻	TUJ1, DCX, MEF2C, NEUN	intermediate progenitors, neurons