Table 2. The oligonucleotide probes used in the PDH model.
| Target microorganism | Probe codea | Sequence (5′ to 3′) | Length (nucleotide) | GC (%) | Tmb (°C) | Locationc | GenBank accession number |
|---|---|---|---|---|---|---|---|
| Acanthamoeba | AC1 | CTGCCACCGAATACATTAGCATGGttttttttttd | 24 | 50.0 | 59.3 | 1145–1168 | KT185626 |
| AC2 | GATTAACTTCTGCGAAAGCATCTtttttttttt | 23 | 39.1 | 51.7 | 1302–1324 | KT185626 | |
| Microsporidia | MS1 | GATGAAGGACGAAGGCTGGAGtttttttttt | 21 | 57.1 | 55.2 | 594–614 | XR552277 |
| MS2 | TCTGGGGATAGTATGCTCGCAAGtttttttttt | 23 | 52.2 | 57.2 | 712–734 | XR552277 |
aOligonucleotide probes are positioned on the PDH model as indicated in Figure 1. bTm, melting temperature. cThe corresponding locus of the probe to the specified GenBank accession number dSeveral bases of thymine were added to the 5′ end of the probe to increase hybridization signal.