Table 3. Positive clinical samples determined by the standard microbiological methods, PCR, and the PDH model, and identification of the infectious agents by DNA sequencing.
| Samples no. | Microscopy |
Culture | Pathology | PCRa | PDH model | Species of the infectious microorganismb | Sequence similarity, % | |
|---|---|---|---|---|---|---|---|---|
| GSc | AFSd | |||||||
| Acanthamoeba (+) | ||||||||
| 1e | − | − | + | NDe | + | + | Acanthamoeba quina | 99.3 |
| 1f | − | − | − | + | − | − | NAf | NA |
| 1h | − | − | − | + | + | + | Acanthamoeba polyphaga | 99.3 |
| 2a | − | − | + | ND | + | + | Acanthamoeba sp. | 92.5 |
| 2d | − | − | + | ND | + | + | Acanthamoeba castellanii | 100 |
| 2g | − | − | + | ND | + | + | Acanthamoeba castellanii | 98.8 |
| 4g | − | − | + | ND | + | + | Acanthamoeba castellanii | 98.8 |
| 4h | − | − | + | ND | + | + | Acanthamoeba castellanii | 98.6 |
| Microsporidia (+) | ||||||||
| 1a | − | − | − | ND | + | + | Vittaforma corneae | 99.2 |
| 1b | + | + | − | ND | + | + | Vittaforma corneae | 100 |
| 1c | + | − | − | ND | + | + | Vittaforma corneae | 100 |
| 1d | + | − | Streptococcus pneumoniae | ND | + | + | Vittaforma corneae | 99.0 |
| 2f | − | − | − | ND | + | + | Vittaforma corneae | 99.3 |
| 2h | − | − | − | ND | + | + | Vittaforma corneae | 99.5 |
| 2j | + | − | − | ND | + | + | Vittaforma corneae | 99.9 |
| 3a | ND | + | − | ND | + | + | Vittaforma corneae | 99.9 |
| 4b | + | − | − | ND | + | + | Vittaforma corneae | 99.9 |
| 4c | + | − | − | ND | + | + | Vittaforma corneae | 99.9 |
| 4d | + | − | − | ND | + | + | Vittaforma corneae | 97.0 |
| 4e | − | − | − | ND | + | + | Vittaforma corneae | 99.6 |
| 4l | + | + | − | ND | + | + | Vittaforma corneae | 99.7 |
aPCR, polymerase chain reaction. bThe species names of the infectious agents were determined by gene sequencing. cGS, Gram stain. dAFS, acid fast stain. eND, not done. fNA, not available.