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Generation of the Nkx2-5/CreER model. (a) Schematic of the Nkx2-5 wild-type allele, gene targeting construct and resultant mutant alleles before (Nkx2-5IRES-CreER-pgkHYGRO) and after (Nkx2-5IRES-CreER) flp-mediated deletion. (b) DNA gel electrophoresis confirming the presence of the 650 bp Nkx2-5CreER band. Lanes 1 and 2: Nkx2-5CreER/+, Lanes 3 and 4: WT control.
