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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Genesis. 2017 Jun 15;55(8):10.1002/dvg.23041. doi: 10.1002/dvg.23041

FIG. 2.

FIG. 2

Characterization of Nkx2-5/CreER expression. (a) Schematic of cross-breeding of Nkx2-5CreER/+ with Rosa26tdT/+ mice. (b) Timeline of 4-OHT injection in E9.5 Nkx2-5CreER/+;Rosa26tdT/+ for IHC analysis at E16.5 and qPCR at P2. (c) E16.5 hearts and P3 hearts were analyzed for tdTomato expression in the absence of 4-OHT administration. There was no visible CreER activity. (d) High tdTomato expression is observed in the embryonic heart of mice labeled at E9.5 and analyzed at E16.5. Inset: shows co-localization of Nkx2-5 staining with tdTomato+ cells. (e) qPCR analysis was performed on tdTomato+ sorted cells from P2 hearts showing almost a 3-fold increase in Nkx2-5 expression compared to blood cells. Values are means ± SEM; *P < 0.05, Scale bar = 500 μm.