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. Author manuscript; available in PMC: 2017 Aug 24.

Published in final edited form as: J Am Chem Soc. 2017 Aug 10;139(33):11434–11442. doi: 10.1021/jacs.7b03966

(A) Overlay of G. stearothermophilus (Gs) EndoIII (magenta, PDB 1ORN) and Gs MutY (cyan, PDB 5DPK), with [Fe₄S₄] cluster (orange for S and yellow for Fe atoms) and key Arg residues highlighted (red and blue respectively). Bound DNA in both structures are in green. Comparison of Ec EndoIII without DNA (pink, PDB 2ABK) in (B) and Gs EndoIII (magenta, PDB 1ORN) bound to DNA (green) in (C) showing the molecular surroundings of the [Fe₄S₄] cluster, including critical Arg residues (red). This comparison highlights the overall structure similarity between Gs EndoIII in complex with DNA to that of the Ec homolog without DNA. Small structural differences (RMSD₁₀₀=1.4 Å) between the two homologs are attributed to their 43% sequence similarity.²⁴

(A) Overlay of G. stearothermophilus (Gs) EndoIII (magenta, PDB 1ORN) and Gs MutY (cyan, PDB 5DPK), with [Fe₄S₄] cluster (orange for S and yellow for Fe atoms) and key Arg residues highlighted (red and blue respectively). Bound DNA in both structures are in green. Comparison of Ec EndoIII without DNA (pink, PDB 2ABK) in (B) and Gs EndoIII (magenta, PDB 1ORN) bound to DNA (green) in (C) showing the molecular surroundings of the [Fe₄S₄] cluster, including critical Arg residues (red). This comparison highlights the overall structure similarity between Gs EndoIII in complex with DNA to that of the Ec homolog without DNA. Small structural differences (RMSD₁₀₀=1.4 Å) between the two homologs are attributed to their 43% sequence similarity.²⁴