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. 2017 May 7;77(9):1114–1129. doi: 10.1002/dneu.22499

Figure 2.

Figure 2

pgrn‐a‐targeted morpholino oligonucleotides inhibit Pgrn‐a translation, which results in microphthalmia. (A) Western blot showing Pgrn‐a and Actin expression in 72 hpf uninjected (UI) and morphant (5′UTR MO) embryos. (B) Histogram showing quantification of Pgrn‐a expression in UI controls (26.2 ± 2.05%) and morphants (2.7 ± 0.9%); **p ≤ 0.01. Quantitative data from three biological and technical replicates is normalized to Actin and represented as mean; error bars represent the standard deviation. (C–H) Representative whole embryos from control (C–F) and experimental (G–H) groups at 72 hpf. 5′UTR MM MO and SS MM MO, embryos injected with 5‐nucleotide mismatch control morpholinos; 5′UTR MO, embryos injected with pgrn‐a 5′UTR‐targeted morpholinos; SS MO, embryos injected with pgrn‐a e3i3 splice site‐targeted morpholinos.