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. 2017 Aug 23;7:9194. doi: 10.1038/s41598-017-09321-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis of selected proteins. HepG2 cells were not treated (mock), treated with 0.3% DMSO or treated with NVP at concentrations ranging from 3.37 μM to 819 μM. On day 7 of treatment cells lysates were prepared which were subjected to SDS-PAGE and western blot analysis to detect the expression of actin, voltage dependent anion channel (VDAC), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), peroxiredoxin-1 (PRDX1), Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1), ATP synthase subunit beta, mitochondrial (ATP5B) and vinculin. The experiment was undertaken independently in triplicate. Protein band intensities were quantitated using the imageJ image analysis program and analyzed by GraphPad Prism 5 program and the expression all proteins was normalized to vinculin. Error bars show S.E.M.