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. 2017 Aug 23;7:9176. doi: 10.1038/s41598-017-09529-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of U0126 inhibitor treatment on cyclin E and CDK2 expression and on viral replication. HEp-2 cells treated or untreated cells with U0126 (5 µM) were mock-infected or infected with HSV-1 at MOI 10 and collected at 3, 9 and 24 h p.i. (a) The cytoplasmic and nuclear proteins extraction were obtained as described in Methods. Equal amount of cytoplasmic and nuclear proteins were separated by polyacrylamide gel electrophoresis and probed with phospho-cyclin E, phospho-CDK (T14-Y15) and Us11 antibodies. The β actin and Histone H3 were used as a loading control. (b) Virus yield was determined at 24 h pi by using the standard plaque assay on VERO cells. The data are shown as means of triplicate ± SD and *indicate significant changes (p value ≤0.001).