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. 2017 Aug 23;7:9176. doi: 10.1038/s41598-017-09529-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cyclin E and CDK2 expression in dominant negative-ERK cells during HSV-1 replication. HEp-dnERK and HEp-2 cells were exposed to HSV-1 at MOI 10 and collected at 3, 9 and 24 h p.i. Cells were processed for cytoplasmic and nuclear proteins extraction as described in Methods and were analyzed by immuno-blot for the expression of total protein cyclin E, phospho-cyclin E, phospho-CDK2 (T160), Us11 and ICP0 proteins. β-actin and Histone H3 were used as cytoplasmic and nuclear loading controls, respectively. The filters derived from extracts obtained from both HEp-dnERK and HEp-2 cell lines were exposed simultaneously.