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. 2017 Aug 23;7:9176. doi: 10.1038/s41598-017-09529-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation between FLAG-dnERK mutant/DNA-content in the infected cells. HEp-dnERK cells, containing an N-terminal FLAG tag in the ERK1 CDS, were mock infected or infected with HSV-1 at MOI 10 and collected at 24 h pi. The cell cycle phase of the FLAG-dnERK mutant cells with/without HSV-1 infection was determined by FACS analysis as described in Methods. Dual-parameter FLAG-dnERK mutant/DNA-content analysis were analyzed using Flowing Software 2.5.0. The percentages of cells distributed in S, G1, G2/M phases are shown in FLAG-dnERK mutant-positive cells. HEp-2 wild type cells not expressing FLAG-dnERK were used as a control to setting instrument. The data are shown as means of triplicate ± SD.