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. 2017 Aug 23;7:9226. doi: 10.1038/s41598-017-10044-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tumor and Chor-IN-1 cell line characterization by H&E and IHC. (a) The original tumor sample and (b) the derived Chor-IN-1 cell line were characterized by H&E, revealing the typical physaliferous cells, and by IHC, showing positivity for brachyury and for other chordoma typical biomarkers, as indicated. In (a), arrows and stars indicate examples of tumor and fibroblast cells, respectively. (c) Growth curve: the doubling time of Chor-IN-1 cell line was calculated as reported and found to be of about seven days. (d) Karyotype analysis of Chor-IN-1 cell line.