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. 2017 Aug 23;7:9226. doi: 10.1038/s41598-017-10044-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of kinases differentially expressed in Chor-IN-1 cell line vs. a panel of chordoma cell lines. (a) Heatmap representing the expression levels of the most differentially expressed kinases in Chor-IN-1 cell line, as obtained by NGS analysis using TREx, Illumina, San Diego, CA, USA. Colormap shades reflect relative low to high expression levels, ranging from green (log2 = 0) to red (log2 = 14). (b) RT-qPCR validation of selected genes (indicated with a star in the heatmap). Histogram bars represent RT-qPCR relative quantification results, carried out as described in M&M and normalized using U-CH1 as a reference sample.