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. 2017 Aug 23;7:9230. doi: 10.1038/s41598-017-08043-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Figure 5A: Evolutionary rate of argonaute transcript sequences within the Caenorhabditis genus. Ka/Ks values are estimated by means of ML-tree based comparison and by using sequence information of C. elegans, C. briggsae, C. remanei and C. brenneri. Figure 5B: The evolutionary rate for Argonaute proteins in nematodes, determined by estimating the Ka/Ks values by means of ML-tree based comparison and by using sequence information of C. elegans, Ascaris suum, Brugia malayi and Necator americanus. Due to the enormous complexity and variation in the Argonaute AGO family present in nematodes, we could only find orthologs of the miRNA-class AGO alg1 and the siRNA-class AGO rde1 in all studied nematode species. The bars represent the mean +/− SEM of individual Ka/Ks values per gene. Statistics (MWU-test) support difference with the housekeeping genes glyceraldehyde 3-phosphate dehydrogenase (gapdh) and apha-tubulin1a (tubu); **p < 0.01, ***p < 0,0001.