Table 1.

Sequence identity and similarity between different metazoan miRNA-class AGOs (upper table) and siRNA-class AGOs (lower table).

Identity-Similarity (%)	Hsa-Ago2	Amis-Ago2	Cel-ALG1	Asu-ALG1	Dme-Ago1	Bmo-Ago1	Ami-Ago1	Nve-Ago1	Aqu-Ago1
Hsa-Ago2	x
Ami-Ago2	94–94	x
Cel-ALG1	60–67	57–65	x
Asu-ALG1	60–68	58–66	79–84	x
Dme-Ago1	64–72	63–69	60–70	63–72	x
Bmo-Ago1	69–77	67–74	61–71	64–72	81–85	x
Ami-Ago1	67–78	65–76	55–65	56–65	58–67	63–72	x
Nve-Ago1	66–77	64–75	54–66	55–66	58–68	63–73	75–84	x
Aqu-Ago1	59–70	55–66	55–66	56–68	58–68	60–71	56–67	56–67	x
Identity-Similarity (%)	Dme-Ago2	Bmo-Ago2	Cel-RDE1	Cel-ERGO1	Asu-ALG5	Asu-ALG7	Ami-Ago2	Nve-Ago2	Aqu-Ago2
Dme-Ago2	x
Bmo-Ago2	23–36	x
Cel-RDE1	13–22	14–29	x
Cel-ERGO1	14–27	14–26	13–26	x
Asu-ALG5	18–29	20–34	19–35	19–36	x
Asu-ALG7	18–29	20–34	20–35	19–33	41–56	x
Ami-Ago2	9–13	10–18	10–18	8–15	9–16	12–20	x
Nve-Ago2	21–33	22–34	15–30	14–26	21–33	20–31	18–23	x
Aqu-Ago2	19–29	23–35	17–31	16–31	27–41	26–39	17–25	29–40	x

The miRNA-class AGO sequences used are of Homo sapiens (chordate, Hsa-Ago2), Alligator mississippiensis (chordate, Ami-Ago2), Caenorhabditis elegans (nematode, Cel-ALG1), Ascaris suum (nematode, Asu-ALG1), Drosophila melanogaster (insect, Dme-Ago1), Bombyx mori (insect, Bmo-Ago1), Acropora millepora (cnidarian, Ami-Ago1), Nemastella vectensis (cnidarian, Nve-Ago1), and Amphimedon queenslandica (sponge, Aqu-Ago1). The siRNA-class AGOs used are of C. elegans (nematode, Ce-RDE1 and Ce-ERGO1), A. suum (nematode, Asu-ALG5 and Asu-ALG7), D. melanogaster (insect, Dme-Ago2), B. mori (insect, Bmo-Ago2), A. millepora (cnidarian, Ami-Ago2), N. vectensis (cnidaria, Nve-Ago2) and A. queenslandica (sponge, Aqu-Ago2). In each cell, the first number corresponds to the percentage of sequence identity, while the second value stands for the percentage of sequence similarity.