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. 2017 Aug 23;7:9260. doi: 10.1038/s41598-017-08932-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Arabidopsis NOG1-1-RNAi but not nog1-2 plants are compromised in nonhost resistance. (A,B) Arabidopsis wild type (Col-0), nog1-2 knockdown line, NOG1-1-RNAi, nog1-2 NOG1-1-RNAi double-gene knockdown lines, overexpression (NOG1-1-OE) and complementation lines (NOG1-2-comp) were flood-inoculated with Pstab (1.4 × 10⁶ CFU/ml) (A) or Psm (1 × 10⁴ CFU/ml) (B) to assess disease symptoms (upper panel) and bacterial growth (lower panel) at 1 and 3 days post inoculation (dpi). Different letters above bars indicate a statistically significant difference within a time point using two-way ANOVA (P < 0.01). Error bars represent the standard deviation of three biological replications (three technical replicates for each biological replication). All experiments were conducted using T2 lines.