Table 1.
Level of cytokines and chemokines in the heart and plasma after TAC.
| Protein | WT | TLR2−/− | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Baseline | Day 7 | Day 14 | Day 21 | Day 28 | Baseline | Day 7 | Day 14 | Day 21 | Day 28 | |
| Cytokines and chemokines in the heart (pg/mg) | ||||||||||
| MCP-1 | 27 ± 7 | 183 ± 127 | 37 ± 2 | 39 ± 4 | 41 ± 7 | 20 ± 4 | 56 ± 7 | 29 ± 5 | 25 ± 2§ | 26 ± 2* |
| IL-1α | 18 ± 5 | 22 ± 3 | 25 ± 1 | 36 ± 1 | 36 ± 7 | 24 ± 2 | 17 ± 3 | 20 ± 4 | 18 ± 3* | 27 ± 6 |
| IL-2 | 37 ± 14 | 32 ± 4 | 39 ± 3 | 64 ± 8 | 42 ± 8 | 27 ± 4 | 32 ± 8 | 22 ± 5* | 26 ± 6§ | 16 ± 4* |
| IFN-γ | 54 ± 17 | 26 ± 7 | 51 ± 3 | 70 ± 13 | 78 ± 33 | 54 ± 17 | 23 ± 5 | 45 ± 13 | 28 ± 5* | 33 ± 7 |
| MIP-1α | 5 ± 1 | 50 ± 38 | 9 ± 1 | 8 ± 2 | 10 ± 2 | 3 ± 1 | 11 ± 2 | 14 ± 10 | 4 ± 0 | 5 ± 0* |
| MIP-1β | 5 ± 1 | 18 ± 10 | 6 ± 1 | 6 ± 1 | 5 ± 0 | 6 ± 1 | 18 ± 3 | 13 ± 5 | 13 ± 3* | 15 ± 3§ |
| Cytokines and chemokines in the plasma (pg/mL) | ||||||||||
| IL-3 | 29 ± 2 | 29 ± 2 | 30 ± 5 | 36 ± 1 | 27 ± 2 | 28 ± 4 | 23 ± 3 | 16 ± 2 | 24 ± 3§ | 21 ± 3 |
| IL-12p40 | 97 ± 7 | 124 ± 11 | 122 ± 12 | 110 ± 10 | 100 ± 15 | 113 ± 10 | 87 ± 6* | 79 ± 11* | 99 ± 8 | 98 ± 7 |
| IL-12p70 | 204 ± 20 | 195 ± 38 | 195 ± 38 | 246 ± 13 | 188 ± 17 | 189 ± 27 | 149 ± 20 | 101 ± 15* | 153 ± 19§ | 135 ± 20 |
| G-CSF | 45 ± 4 | 46 ± 4 | 42 ± 4 | 59 ± 7 | 38 ± 2 | 41 ± 9 | 30 ± 3* | 22 ± 3* | 31 ± 3# | 39 ± 7 |
| KC | 27 ± 3 | 41 ± 10 | 33 ± 4 | 38 ± 3 | 27 ± 3 | 25 ± 5 | 34 ± 5 | 18 ± 4* | 26 ± 1§ | 34 ± 6 |
| MIP-1β | 38 ± 3 | 38 ± 4 | 34 ± 5 | 37 ± 3 | 30 ± 3 | 39 ± 3 | 42 ± 4 | 33 ± 4 | 41 ± 6 | 29 ± 4 |
| RANTES | 6 ± 1 | 7 ± 0 | 7 ± 1 | 7 ± 0 | 6 ± 1 | 5 ± 1 | 4 ± 0* | 3 ± 1* | 4 ± 0§ | 5 ± 0 |
| TNF-α | 427 ± 24 | 395 ± 21 | 393 ± 52 | 451 ± 27 | 333 ± 25 | 394 ± 29 | 422 ± 53 | 291 ± 36* | 457 ± 67 | 267 ± 46 |
| IL-6 | 19 ± 10 | 11 ± 1 | 8 ± 1 | 12 ± 1 | 8 ± 1 | 14 ± 3 | 9 ± 1 | 7 ± 1 | 8 ± 1* | 8 ± 1 |
Mouse hearts and plasma were obtained at indicated timepoints for measurement of protein levels with multiplex assays. * p < 0.05, § p < 0.01, # p < 0.001, non-parametric test compared to WT mice at respective timepoints. N = 6–8 per timepoint per genotype of mice. MCP-1, monocyte chemoattractant protein-1; IL-1α, interleukin 1 alpha; IL-2, interleukin 2; IL-3, interleukin 3; IL-6, interleukin 6; IFN-γ, interferon gamma; MIP-1α, macrophage inflammatory protein 1 alpha; MIP-1β, macrophage inflammatory protein 1 beta; IL-12p40, interleukin-12 p40; IL-12p70, interleukin-12 p70; G-CSF, granulocyte-colony stimulating factor; KC, keratinocyte chemoattractant; RANTES, Regulated on Activation, Normal T Cell Expressed and Secreted, also known as chemokine (C-C motif) ligand 5 (CCL5); TNF-α, tumor necrosis factor-alpha.