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. 2017 Aug 23;7:9223. doi: 10.1038/s41598-017-10087-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Oxidative stress response in persistently HCV infected Huh-7.5 cells. (A) Oxidative stress due to generation of ROS was measured using conventional fluorescence microscopy. In this assay, the nonfluorescent dye (H2DCFDA) is oxidized to a fluorescence green (DFA) by ROS only in HCV infected cells. Uninfected cells show no fluorescence. (B) Quantification of fluorescent intensity in three different areas of uninfected and infected culture was measured by ImageJ software. Fluorescence values are proportional to intracellular ROS. (C) Flow cytometric analysis of oxidized fluorescence of uninfected and infected Huh-7.5 cells. (D) Relative Oxidation-Reduction Potential (ORP) of cell free media of uninfected and HCV infected Huh-7.5 cells.