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. 2017 Aug 23;7:9209. doi: 10.1038/s41598-017-08957-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The morphological integrity of neurons is rescued by CE. Immunofluorescence staining was performed using an anti-MAP2 antibody, followed by an Alexa Fluor® 546-conjugated secondary antibody. Nuclei were co-immunostained with Hoechst 33258. Images were acquired using a Leica confocal microscope. Three independent experiments were performed; at least 20 photomicrographs from each group were analysed. Scale bar, 50 μm.