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. 2017 Aug 23;7:9195. doi: 10.1038/s41598-017-09697-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Statistics of early tumorigenesis in embryos with constitutive activation of kRasG12V. Tg(ubi:Cre-ERT2) embryos injected with ubi:loxP-Eos-stop-loxP-kRASG12V-T2A-mTFP plasmid were split into cyclofen, no cyclofen, caged cyclofen + UV and caged cyclofen groups. Tumorigenic probabilities were measured for each group from 3 dpf to 10 dpf (A). For the cyclofen group, embryos were separated into four sub-groups based on the number of cells expressing mTFP at 3dpf (more or less than 50 (B) and more or less than 100 cells (C)), and tumorigenic probabilities were assessed for each sub-group (B,C). Statistical significance: **p < 0.01.