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. 2017 Aug 23;7:9195. doi: 10.1038/s41598-017-09697-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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KRASG12V activation and tracking in a small group of cells within a live zebrafish embryo. A stable Tg(ubi:Eos;UAS:kRASG12V-T2A-CFP) line was generated and crossed with the Tg(ubi:Gal4-ERT2) line. Caged cyclofen alone at 1 dpf was not able to induce CFP expression (A), while 2-minute UV illumination (B) effectively turned on CFP after 18 hours. (C) Local activation of kRASG12V expression was achieved by introducing a focalized UV beam onto embryos pre-incubated with caged cyclofen. (D,E) 1 minute UV effectively converted EosFP from green to red. (F,G) After 18 hours, CFP expressed in a localized region of the illuminated embryo and weak remaining EosFP red signal was detected in the same region (H). Scale bar: 200 μm.